This novel strategy for Biotech/ Biosimilar products without using any antibiotics throughout the process, can serve mankind in the following ways:

• In the current scenario, Biopharma and Biotechnology industries are discouraged by regulatory for use of antibiotics in their process.
• Unused antibiotics, after disposal of the culture media is a potential reason to generate multi drug resistant (MDR) strains in the environment.
• As per regulatory requirement, antibiotic clearance needs to be shown, which increases product cost. Hence, antibiotic free process will lead to a cost effective product.
• In our strategy, chance of loss of plasmid copy number is nil, so yield of expression is high and consistency of expression from batch to batch